Journal
ANALYTICAL CHEMISTRY
Volume 88, Issue 5, Pages 2884-2890Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.5b04716
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Funding
- 973 Program [2012CB932600, 2013CB933802]
- National Natural Science Foundation of China [21327902, 21535003]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
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In this work, we developed a visual electrochemiluminescence (ECL) sensing platform based on a dual-bipolar electrode (D-BPE) array chip. The chip was composed of two arrays of BPEs and three separated arrays of reservoirs filled with buffer, Ru(bpy)(3)(2+)-TPrA and luminol solutions, respectively. Both BPEs served as ECL reporting platforms. By applying 6.0 V voltage, an array of orange electrochemiluminescence (ECL) signals belonging to Ru(bpy)(3)(2+) turned on. After adding DNAzyme and H2O2 in Ru(bpy)(3)(2+) and luminol reservoirs, the orange Ru(bpy)(3)(2+) signals decreased until vanished due to the quenching effect; meanwhile, a new array of blue ECL signals turned on because of the luminol-H2O2 ECL reaction. The designed D-BPE owns superior properties compared with the three-electrode system benefiting from the quantitative relation of bipolar systems, which greatly enhanced the ECL detection sensitivity. Meanwhile, the visual color-switch ECL and the ratiometric detecting principle made the results easier to evaluate and more accurate. Quantitative detection of HL-60 cancer cells from 320 cells/mL to 2.5 x 10(5) cells/mL with two linear ranges was achieved. The detection limit was down to 80 cells/mL. Finally, this D-BPE chip could distinguish the tumor cells from normal cells and provide a prospect for cancer diagnosis in a high-throughput, visual way.
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