Journal
ANALYTICAL CHEMISTRY
Volume 88, Issue 8, Pages 4318-4327Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.5b04471
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Funding
- National Natural Science Foundation of China [61071002]
- National Program for Significant Scientific Instruments Development of China [2011YQ030134]
- Funds of State Key Laboratory of China
- Scientific Research Foundation for Returned Overseas Chinese Scholars
- Funds for Beijing Laboratory of Biomedical Detection Technology and Instrument
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Quantum dot (QD) multicolor analysis was achieved by labeling the epidermal growth factor and labeling the antibody against the epidermal growth factor receptor (anti-EGFR) using different QDs. On the basis of the fluorescence intensity of the two types of QDs, the amount of epidermal growth factors (EGFs) in the cells and anti-EGFRs bound to EGFRs were analyzed. The functions of anti-EGFR in preventing HeLa cells from engulfing EGF and inhibiting overproliferation of the HeLa cells were also studied. Meanwhile, parallel analysis was conducted to analyze the heterogeneity of cells at the single-cell level using a single-cell array. This provides a novel approach for the multiplexed analysis for the anti-EGFR functions in cells together with the cell heterogeneity. It also lays a foundation for parallel analysis and detection, using various fluorescence probes, simultaneous tracking, and detection of multiple targets at an overall level or individual level, and multichannel analysis of drug effects.
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