Journal
ANALYTICAL CHEMISTRY
Volume 88, Issue 21, Pages 10540-10546Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.6b02620
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Funding
- National Natural Science Foundation of China [21405073, 21535002]
- Innovation Team Development Plan of the Ministry of Education Rolling Support [IRT_15R31]
- Open Funds of the State Key Laboratory of Electroanalytical Chemistry [SKLEAC201502]
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The accurate discrimination of microRNAs (miRNAs) with highly similar sequences would greatly facilitate the screening and early diagnosis of diseases. In the present work, a locked nucleic acid (LNA)-modified probe was designed and used for a-hemolysin (alpha-HL) nanopore to selectively and specifically identify miRNAs. The hybridization of the LNA probe with the target miRNAs generated unique long-lived signals in the nanopore thus facilitated an accurate discrimination of miRNAs with similar sequences, even a single-nucleotide difference. Furthermore, the developed nanopore-based analysis with LNA probe could selectively detect target miRNAs in a natural serum background. This selective and sensitive approach may be highly valuable in the detection of clinically relevant biomarkers in complex samples.
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