Journal
ANALYTICAL CHEMISTRY
Volume 88, Issue 7, Pages 3864-3871Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.6b00012
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Funding
- 973 Program [2012CB932600]
- National Natural Science Foundation of China [21327902, 21135003, 21305063]
- Natural Science Funds of Jiangsu Province [BK20130553]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
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In this work, we present a novel energy-transfer (ET)-based photoelectrochemical (PEC) probing of DNA protein interactions, which associates intimately with many important intracellular processes in transcriptional regulatory networks. Specifically, Au nanoparticles (NPs) were confined onto the CdS quantum dots (QDs) functionalized PEC surface by the formation of duplex DNA, the subsequent binding of the TATA binding protein (TBP) and the resulting distortion of the Au NPs capped DNA sequence could adjust the interparticle distance and thereby modulate the PEC performance of CdS QDs through the ET process between the CdS QDs and Au NPs. Using the duplex DNA sequence as a rigid spacer, the relationship between the photocurrent quenching effect and the spacing distance was also studied and some experimental conditions were optimized, on the basis of which a novel ET-based PEC TBP biosensor was realized with high sensitivity and selectivity.
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