4.8 Article

Sequential Collision- and Ozone-Induced Dissociation Enables Assignment of Relative Acyl Chain Position in Triacylglycerols

Journal

ANALYTICAL CHEMISTRY
Volume 88, Issue 5, Pages 2685-2692

Publisher

AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.5b04001

Keywords

-

Funding

  1. Science and Engineering Faculty (QUT)
  2. Australian Research Council (ARC) [DP150101715]
  3. ARC
  4. SCIEX (Concord, Ontario, Canada) [LP110200648]
  5. Singapore National Research Foundation [NRF-RF2010-06]

Ask authors/readers for more resources

Unambiguous identification of isomeric lipids by mass spectrometry represents a significant analytical challenge in contemporary lipidomics. Herein, the combination of collision-induced dissociation (CID) with ozone induced dissociation (OzID) on an ion-trap mass spectrometer is applied to the identification of triacylglycerol (TG) isomers that vary only by the substitution pattern of fatty acyl (FA) chains esterified to the glycerol backbone. Isolated product ions attributed to loss of a single FA arising from CID of [TG + Na](+) ions react rapidly with ozone within the ion trap. The resulting CID/OzID spectra exhibit abundant ions that unequivocally reveal the relative position of FAs along the backbone. Isomeric TGs containing two or three different FA substituents are readily differentiated by diagnostic ions present in their CID/OzID spectra. Compatibility of this method with chromatographic separations enables the characterization of unusual TGs containing multiple short-chain FAs present in Drosophila.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.8
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available