Journal
NATURE NANOTECHNOLOGY
Volume 14, Issue 5, Pages 456-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/s41565-019-0382-5
Keywords
-
Funding
- Burroughs Wellcome Fund Career Award at the Scientific Interface (CASI)
- Stanley Fahn PDF Junior Faculty Grant [PF-JFA-1760]
- Beckman Foundation Young Investigator Award
- USDA AFRI award
- Gordon and Betty Moore Foundation
- USDA NIFA award
- Chan-Zuckerberg foundation
- FFAR New Innovator Award
- Schlumberger Foundation Faculty for the Future Fellowship
- National Defense Science and Engineering Graduate (NDSEG) Fellowship
- LAM Foundation
- UC Berkeley Molecular Imaging Center (Gordon and Betty Moore Foundation)
- UC Berkeley Biological Imaging Facility (National Institutes of Health S10 program) [1S10OD018136-01]
- Innovative Genomics Institute (IGI)
- QB3 Shared Stem Cell Facility
Ask authors/readers for more resources
Genetic engineering of plants is at the core of sustainability efforts, natural product synthesis and crop engineering. The plant cell wall is a barrier that limits the ease and throughput of exogenous biomolecule delivery to plants. Current delivery methods either suffer from host-range limitations, low transformation efficiencies, tissue damage or unavoidable DNA integration into the host genome. Here, we demonstrate efficient diffusion-based biomolecule delivery into intact plants of several species with pristine and chemically functionalized high aspect ratio nanomaterials. Efficient DNA delivery and strong protein expression without transgene integration is accomplished in Nicotiana benthamiana (Nb), Eruca sativa (arugula), Triticum aestivum (wheat) and Gossypium hirsutum (cotton) leaves and arugula protoplasts. We find that nanomaterials not only facilitate biomolecule transport into plant cells but also protect polynucleotides from nuclease degradation. Our work provides a tool for species-independent and passive delivery of genetic material, without transgene integration, into plant cells for diverse biotechnology applications.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available