Journal
ANALYTICAL CHEMISTRY
Volume 89, Issue 1, Pages 745-750Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.6b03328
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Funding
- National Key Research and Development Plan of China [2016YFC0100904]
- China Agriculture Research System Poultry-related Science and Technology Innovation Team of Peking (CARS-PSTP)
- National Natural Science Foundation of China [31470221]
- National Wildlife-borne Diseases Surveillance Program of State Forestry Administration of China
- External Cooperation Program of BIC/Chinese Academy of Sciences [152111KYS820150023]
- Chinese Academy of Sciences [CZBZX-1, KSCX2-YW-Z-1031]
- Beijing Municipal Science & Technology Commission [Z161100000116042]
- Scientific and Technology Development Program of Hangzhou [20131813A07]
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Human infection= with avian influenza A HSN1-viruses can cause severe diseases with high mortality rate and, continues to pose a significant threat to global public health. Rapid diagnosis is needed for identifying the types of influenza viruses for making timely treatment decisions. Here, we demonstrate absolute quantification of HS-subtype influenza viruses-by digital loop-thediated isothermal amplification (dLAMP) on our recently-developed cross-interface emulsification (XiE) method. Our results show that XiE-based dLAMP is, highly specific and displays comparable sensitivity to real-time PCR (qPCR) and digital FCR. (dPCR). Notably,.dLAMP is more tolerant to inhibitOry substanCes than PCR methods and demonstrated similar detection efficiency to qPCR for real _HSN1,samples. Therefore, it can serve as a robust and precise alternative to qPCR or dPCR and is especially suitable for en-vironmentaLand-clinical-samples with hard-to-remove contaminants. We believe that our dLAMP method offers great potential for rapid and :accurate diagnosis of influenza, and other infectious diseases.
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