Journal
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
Volume 16, Issue -, Pages 69-78Publisher
ELSEVIER
DOI: 10.1016/j.nano.2018.11.009
Keywords
Ferritin nanoparticle; SpyTag/SpyCatcher; Click vaccine; Therapeutic tumor vaccine; Neoantigen
Funding
- Strategic Priority Research Program of the Chinese Academy of Sciences [XDB29040202]
- National Natural Science Foundation of China [31570888]
Ask authors/readers for more resources
Recently, tumor neoantigens have been attractive for development of personal therapeutic vaccines. However, how to instantly deliver multiple neoantigens for efficient anti-tumor immunity is still challenging. Here, we established a SpyCatcher-modified ferritin nanoparticle platform, which permits convenient and stable covalent conjugation with tumor specific antigens containing SpyTag in a click-link manner These ferritin nanoparticles are rapidly drained to lymph nodes and target dendritic cells, especially CD8 alpha(+) population, upon subcutaneous immunization. Ferritin nanoparticles carrying HPV16 oncogene E7 peptide antigen or MC38 tumor derived mutant neoantigens elicit about 2-3 folds enhanced antigen-specific cytotoxic T lymphocyte (CTL) response than soluble peptide antigens and significantly suppress the growth of E7-related or MC38 tumors. The anti-tumor effect was further enhanced in combination with PD-1 checkpoint blockade. Together, our study provides a ferritin nanoparticle-based, SpyTag/SpyCatcher-enabled click vaccine platform, especially for personalized minor immunotherapy. (C) 2018 Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available