Journal
MOLECULES
Volume 24, Issue 4, Pages -Publisher
MDPI
DOI: 10.3390/molecules24040762
Keywords
wedelolactone; pharmacokinetics; UPLC; rat plasma
Funding
- National Natural Science Foundation of China [31300285]
- Natural Science Foundation of Fujian Province [2015J01348]
- Specialized Research Fund for the Doctoral Program of Higher Education [20110073120072]
- Fujian Province Development Guidance (Key) Project [2014Y0074]
- Educational and Scientific Research Project for Young and Middle-aged Teachers of Fujian Province [JT180012]
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Wedelolactone is a coumarin ether with significant hepatoprotective effects. However, there are few pharmacokinetic studies of wedelolactone, which will affect the studies of its efficacy and potential toxicity. In this study, a selective ultra-performance liquid chromatography (UPLC) method was developed to confirm the pharmacokinetic parameters of wedelolactone in rat plasma. The chromatographic separation was carried out on a Kromasil C-18 UPLC column (250 x 4.6 mm; 5.0 m) by gradient mobile phase of methanol-water containing 0.5% acetic acid (v/v). Perfect linearity was obtained and the samples were stable under different conditions. The intra-day and inter-day precisions (relative standard deviation, %) were within 3.81% and accuracies (relative error, %) ranged from -4.01% to 7.12%. The extraction recoveries in rat plasma ranged from 95.98% to 108.93%. This rapid method was successfully applied in the pharmacokinetic study of wedelolactone in rat plasma. Following the oral administration of 5.00 mg/kg wedelolactone, the wedelolactone was rapidly absorbed. Pharmacokinetic parameters were used to quantitatively describe the dynamic changes of wedelolactone in vivo, providing a theoretical basis for pharmacological research on drugs and preclinical medication. The study of wedelolactone can provide a theoretical basis and quick analysis for the study of other traditional Chinese medicine. This may lead to breakthroughs in the pharmacokinetic study of complex Chinese medicines.
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