4.6 Article

Urinary Proteomics Profiles Are Useful for Detection of Cancer Biomarkers and Changes Induced by Therapeutic Procedures

Journal

MOLECULES
Volume 24, Issue 4, Pages -

Publisher

MDPI
DOI: 10.3390/molecules24040794

Keywords

urine; thyroid cancer; squamous cell cancer of head and neck; BNCT; boron; proteomics; LC-MS; MudPIT

Funding

  1. Italian CNR FLAGSHIPs Project - MIUR InterOmics
  2. AMANDA project - CNR-Lombardy Agreement
  3. European Commission [QLK3-CT-1999-01067, 005045]
  4. Fondazione Umberto Veronesi Fellowship

Ask authors/readers for more resources

Boron neutron capture therapy (BNCT) is a binary cancer treatment modality where two different agents (B-10 and thermal neutrons) have to be present to produce an effect. A dedicated trial design is necessary for early clinical trials. The concentration of B-10 in tissues is an accepted surrogate to predict BNCT effects on tissues. Tissue, blood, and urines were sampled after infusion of two different boron carriers, namely BSH and BPA in the frame of the European Organisation for Research and Treatment of Cancer (EORTC) trial 11001. In this study, urine samples were used to identify protein profiles prior and after drug infusion during surgery. Here, an approach that is based on the mass spectrometry (MS)-based proteomic analysis of urine samples from head and neck squamous cell carcinoma (HNSCC) and thyroid cancer patients is presented. This method allowed the identification of several inflammation- and cancer-related proteins, which could serve as tumor biomarkers. In addition, changes in the urinary proteome during and after therapeutic interventions were detected. In particular, a reduction of three proteins that were involved in inflammation has been observed: Galectin-3 Binding Protein, CD44, and osteopontin. The present work represents a proof of principle to follow proteasome changes during complex treatments based on urine samples.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.6
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available