Journal
ANALYTICAL CHEMISTRY
Volume 88, Issue 17, Pages 8596-8603Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.6b01734
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Funding
- Research Foundation-Flanders [FWO G086114N, G080016N]
- De Vlaamse Liga tegen Kanker [EXM C8744-A]
- Hercules initiative for large equipment (HELICOM) [ZW11-15]
- KU Leuven [OT 13/058, IDO 10/012, IOF KP/12/009]
- EU [FP7-NMP-2013-SMALL-7604244]
- Agency for Innovation by Science and Technology in Flanders [IWT 121615]
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The detection of single molecules in magnetic microbead microwell array formats revolutionized the development of digital bioassays. However, retrieval of individual magnetic beads from these arrays has not been realized until now despite having great potential for studying captured targets at the individual level. In this paper, optical tweezers were implemented on a digital microfluidic platform for accurate manipulation of single magnetic beads seeded in a microwell array. Successful optical trapping of magnetic beads was found to be dependent on Brownian motion of the beads, suggesting a 99% chance of trapping a vibrating bead. A tailor-made experimental design was used to screen the effect of bead type, ionic buffer strength, surfactant type, and concentration on the Brownian activity of beads in microwells. With the optimal conditions, the manipulation of magnetic beads was demonstrated by their trapping, retrieving, transporting, and repositioning to a desired microwell on the array. The presented platform combines the strengths of digital microfluidics, digital bioassays, and optical tweezers, resulting in a powerful dynamic microwell array system for single molecule and single cell studies.
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