Journal
MOLECULAR IMMUNOLOGY
Volume 106, Issue -, Pages 87-98Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.molimm.2018.12.019
Keywords
Duck; TLR21; CpG-ODN; NF-kappa B; DPV
Categories
Funding
- National Natural Science Foundation of China [31872475]
- Sichuan Veterinary Medicine and Drug Innovation Group of China Agricultural Research System (CARS-SVDIP)
- China Agricultural Research System [CARS-42-17]
- Sichuan Province Research Programs [2017JY0014/2017HH0026]
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TLR21 can recognize unmethylated cytosine-phosphate-guanine oligodeoxynucleotides (CpG-ODN) and activates NF-kappa B immune signaling pathway. However, the function of TLR21 in duck remains largely unclear. Here, the complete duck TLR21 (duTLR21) cDNA was cloned from Cherry Valley duck for the first time, and its immune response was preliminarily studied. Tissue specificity analysis showed duTLR21 was higher expressed in the peripheral blood, spleen, bursa of Fabricius and cecum. The expression of duTLR21 was significantly up regulated after stimulation with CpG-ODN or duck plague virus (DPV), but not Tembusu virus (TMUV), LPS or Poly (I:C). In addition, the transfection of DEF with duTLR21 stimulated by CpG-ODN activated NF-kappa B, through this signal pathway, the transcription of IL-1 beta, IL-6 and IFN-alpha were promoted, whereas knockdown of duTLR21 impaired the transcription of these genes. Furthermore, the overexpression of duTLR21 inhibited the replication of the DPV and the knockdown of duTLR21 by shRNA significantly promoted DPV replication in vitro. Altogether, these results indicate that duTLR21 can be activated by CpG-ODN, which mediates activation of NF-kappa B signaling pathway, and plays an important role in the host defence of DPV infection.
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