Journal
ANALYTICAL BIOCHEMISTRY
Volume 494, Issue -, Pages 10-15Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2015.10.008
Keywords
Carbon nanotubes; Poly(diallyldimethylammoniumchloride); Pd-Au nanoparticles; Aflatoxin B1; Immunosensors
Funding
- National Natural Science Foundation of China [21205039]
- Natural Science Foundation of Hunan Province [13JJ6071]
- Open Project of State Key Laboratory of Chemo/Biosensing and Chemometrics [2013004]
- Research Foundation of Education Bureau of Hunan Province
- Doctor's Start-up Foundation of Hunan University of Arts and Science
- Construct Program of Key Discipline in Hunan Province (Applied Chemistry)
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This paper reports a label-free electrochemical immunosensor for the determination of aflatoxin B1 (AFB1), which is based on a gold electrode modified by a biocompatible film of carbon nanotubes/poly(diallyldimethylammoniumchloride)/Pd-Au nanopartides (CNTs/PDDA/Pd-Au). The nanocomposite was characterized by transmission electron microscopy and the electrochemical behavior of modified electrodes was investigated by cyclic voltammetry. The CNTs/PDDA/Pd-Au nanocomposites film showed good electron transfer ability, which ensured high sensitivity to detect AFB1 in a range from 0.05 to 25 ng mL(-1) with a detection limit of 0.03 ng mL(-1) obtained at 3 sigma (where sigma is the standard deviation of the blank solution, n = 10). The proposed immunosensor provides a simple tool for AFB1 detection. This strategy can be extended to any other antigen detection by using the corresponding antibodies. (C) 2015 Elsevier Inc. All rights reserved.
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