Journal
MOLECULAR BREEDING
Volume 39, Issue 3, Pages -Publisher
SPRINGER
DOI: 10.1007/s11032-019-0954-y
Keywords
CRISPR; Cas9; OsRR22; Salinity tolerance; Genetic engineering; Abiotic stress
Categories
Funding
- National High Technology Research and Development Programs of China [2014AA10A603, 2014AA10A604]
- Bill and Melinda Gates Foundation [OPP1130530-4]
- Shanghai Sailing Program [16YF1410000]
- National Natural Science Foundation of China [31501270]
- key program of Shanghai Prospering Agriculture by Science and Technology [2017-02-08-00-08-F00071]
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Salinity is one of the most important abiotic stress affecting the world rice production. The cultivation of salinity-tolerant cultivars is the most cost-effective and environmentally friendly approach for salinity control. In recent years, CRISPR/Cas9 systems have been widely used for target-site genome editing; however, their application for the improvement of elite rice cultivars has rarely been reported. Here, we report the improvement of the rice salinity tolerance by engineering a Cas9-OsRR22-gRNA expressing vector, targeting the OsRR22 gene in rice. Nine mutant plants were identified from 14 T-0 transgenic plants. Sequencing showed that these plants had six mutation types at the target site, all of which were successfully transmitted to the next generations. Mutant plants without transferred DNA (T-DNA) were obtained via segregation in the T1 generations. Two T2 homozygous mutant lines were further examined for their salinity tolerance and agronomic traits. The results showed that, at the seedling stage, the salinity tolerance of T2 homozygous mutant lines was significantly enhanced compared to wild-type plants. Furthermore, no significantly different agronomic traits were found between T2 homozygous mutant lines and wild-type plants. Our results indicate CRISPR/Cas9 as a useful approach to enhance the salinity tolerance of rice.
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