Journal
ANALYTICAL BIOCHEMISTRY
Volume 514, Issue -, Pages 48-54Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2016.09.007
Keywords
Fluorescence; Heparin; Glycosaminoglycans; Detection; Aggregate induce emission
Funding
- National Institutes of Health [HL125371, GM38060, GM103390, GM090127, HL096972, HL10172]
- China Scholarship Council
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A positively charged tetraphenylethene (TPE) derivative, TPE-4MN, was synthesized as a probe for heparin based on aggregation induced emission. On the addition of 5.0 mu g/mL of heparin, TPE-4MN showed an enhanced emission of about 10-fold. The change in fluorescence at 475 nm was linear over a range of heparin concentrations of 0-1.0 mu g/mL with an R = 0.99988 and the limit of detection (LOD) was calculated to be 0.75 mu g/mL. The mechanism of the detection was proven to be through an ion pairing interaction. TPE-4MN showed good selectivity for heparin over other types of polysaccharides and could easily distinguish heparin from heparan sulfate, a glycosaminoglycan having a similar structure to that of heparin. (C) 2016 Elsevier Inc. All rights reserved.
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