Journal
MOLECULAR AND CELLULAR ENDOCRINOLOGY
Volume 482, Issue -, Pages 70-80Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.mce.2018.12.012
Keywords
Spermatogenesis; Androgen receptor; FSH-Receptor; Male fertility; Sertoli cells
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Funding
- INDO-US program on Contraception And Reproductive Health Research (CRHR) of Department of Biotechnology (DBT), Ministry of Science and Technology, India
- MHRD/UGC- Empowered Committee's Basic Science Research (BSR) program, University Grant Commission (UGC), India
- TATA Innovation Fellowship from Department of Biotechnology (DBT), Ministry of Science and Technology, India
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The synergistic actions of Testosterone (T) and FSH via testicular Sertoli cells (Sc) regulate male fertility. We have previously reported that the actions of these hormones (T and FSH) in infant monkey testes are restricted only to the expansion of Sc and spermatogonial cells. The robust differentiation of male Germ cells (Gc) occurs after pubertal maturation of testis. The present study was aimed to investigate the molecular basis of the synergy between T and FSH action in pubertal primate (Macaca mulatta) Sc. Using primary Sc culture, we here have demonstrated that T (but not FSH) downregulated AMH and Inhibin-beta-B (INHBB) mRNAs in pubertal Sc. We also found that, prolonged stimulation of T in pubertal Sc significantly elevated the expression of genes involved in FSH signaling pathway like FSH-Receptor (FSHR), GNAS and RIC8B, and this was associated with a rise in cAMP production. T also augmented FSH induced expression of genes like SCF, GDNF, ABP and Transferrin (TF) in pubertal Sc. We therefore conclude that T acts in synergy with FSH signaling in pubertal Sc. Such a coordinated network of hormonal signaling in Sc may facilitate the timely onset of the first spermatogenic wave in pubertal primates and is responsible for quantitatively and qualitatively normal spermatogenesis.
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