Journal
MOLECULAR AND CELLULAR ENDOCRINOLOGY
Volume 481, Issue -, Pages 62-70Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.mce.2018.11.010
Keywords
Hydrogen peroxide; Thyroid; DNA damage; Antioxidant enzymes
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Funding
- Fonds Erasme pour la Recherche Medicale
- FRS-FNRS: Fonds de la Recherche Scientifique Medicale
- Belgian Kids' Fund
- Association Vincotte Nuclear (AVN)
- Fonds Docteur J.P Naets
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We studied the mechanism that may explain the relative resistance of thyrocytes to H2O2 compared to other cell types. Ability to degrade H2O2, glutathione peroxidase (GPx) activity, heme oxygenase-1 (HO-1) expression, cell survival and capacity to repair DNA damage after H2O2 exposure or irradiation were measured in human thyrocytes in primary culture and compared to the values obtained in human T-cells and different cell lines. Compared to other cell types, thyrocytes presented a low mortality rate after H2O2 exposure, rapidly degraded extracellular H2O2 and presented a high basal seleno-dependent GPx activity. Only in thyrocytes, H2O2 upregulated GPx activity and expression of HO-1 mRNA. These effects were not reproduced by irradiation. DNA damage caused by H2O2 was more slowly repaired than that caused by irradiation and not repaired at all in T-cells. Our study demonstrates that the thyrocyte has specific protective mechanisms against H(2)O(2 )and its mutagenic effects.
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