Journal
MICROCHIMICA ACTA
Volume 186, Issue 3, Pages -Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-019-3314-z
Keywords
Aptamer; Biosensor; DNA; Mycotoxins; Metal nanoparticles; Enzymatic polymerization; Food safety; Terminal deoxynucleotidyl transferase; Structure switching; Red wine
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Funding
- Natural Science Foundation Project of CQ [cstc2018jscx-msybX0263]
- National Natural Science Foundation of China [21405125]
- China Agriculture Research System [CARS-27]
- National Risk Assessment Program for Agricultural Products Quality and Safety [GJFP2018003, GJFP2017013]
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A fluorescence enhancement method is presented for the determination of ochratoxin A (OTA). The interaction of OTA with its aptamer causes structural changes which, in turn, change fluorescence of enzymatically generated polythymine-coated copper nanoparticles (CuNPs) (with excitation/emission maxima at 340/625nm). The OTA-binding aptamer was immobilized on magnetic beads. When it binds OTA, it is partially released and exposes a region with a partly complimentary DNA strand (cDNA). After magnetic separation, the cDNA was employed as a primer to trigger the terminal deoxynucleotidyl transferase-mediated polymerization. This process generates polythymine which act as a template for synthesis of the CuNPs. The method is sensitive in having a 2.0nM detection limit for OTA. It was successfully applied to the determination of OTA in spiked diluted red wine.
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