Journal
MICROCHIMICA ACTA
Volume 186, Issue 4, Pages -Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-019-3352-6
Keywords
Biosensor; Aptamer; Carbon screen-printed electrode; Gold nanoparticles; Ionic liquids; Troponin I; Acute myocardial infarction; Cardiovascular diseases; Human serum analysis
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An ultrasensitive electrochemiluminescence (ECL) disposable aptamer sensor (aptasensor) is presented for detection of myocardial infarction biomarker by quantification of troponin I in blood serum. A screen-printed electrode was modified with (a) aptamer-modified gold nanoparticles, (b) cyclometallated iridium(III)-poly-4-vinylpyridine nanoparticles, and (c) nitrogen-doped graphene in order to increase the loading capacity and conductivity of the aptasensor. If the aptasensor is exposed to troponin I, it will bind to the aptamer and desorb the aptamer from gold nanoparticles and the surface of the electrode. This generates an enhancement in ECL emission depending on troponin I concentration. ECL emission is strongly improved by aggregation-induced phenomenon, which is caused by inhibition of the water and oxygen quenching effect on the iridium complex ECL in aqueous media. Under optimum conditions, the aptasensor has a wide dynamic range that extends from 0.1 pM to 10nM, with a 20 fM detection limit (S/N=3) and a relative standard deviation of 3.1%. The ECL aptasensor was successfully applied to 20 individual human serum for the detection of troponin I biomarker.
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