Journal
MICROCHIMICA ACTA
Volume 186, Issue 4, Pages -Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-019-3322-z
Keywords
Ultrasensitive; Silver nanoclusters (AgNCs); Fluorescence; Wide analytical range
Categories
Funding
- National Key Research and Development Program of China [2017YFC1200903, 2017YFF0108403, 2017YFC1601205]
- National Natural Science Foundation of China [81703229, 31701711]
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The authors describe an aptamer based assay for the mycotoxin T-2. The method is making use of exponential isothermal amplification reaction (EXPAR) and fluorescent silver nanoclusters (AgNCs). Free T-2 and cDNA (which is a DNA that is partially complementary to the aptamer) compete for binding to aptamer-modified magnetic beads. The cDNA collected by magnetic separation can be used as a primer to trigger EXPAR to obtain ssDNA. The C-base-rich ssDNA binds and reduces Ag(I) ion to form fluorescent AgNCs. Fluorescence is measured at excitation/emission wavelengths of 480/525nm. T-2 can be detected by fluorometry with a detection limit as low as 30fg mL (-1). The method was applied to analyse spiked oat and corn, and the average recoveries ranged from 97.3 to 102.3% and from 95.9 to 107.5%, respectively. The results were in good agreement with data of the commercial ELISA kit. The assay is highly sensitive, has a wide analytical range, good specificity and reliable operation. It provides a promising alternative for the standard method for quantitative detection of T-2.
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