Journal
MICROCHEMICAL JOURNAL
Volume 145, Issue -, Pages 864-871Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.microc.2018.11.059
Keywords
Metal-free polymer nanozyme; Peroxidase-like activity; Chromogenic reaction; Electrostatic attraction; Heparin
Categories
Funding
- National Natural Science Foundation of China [21675099, 21375075]
- Major Basic Research Program of Natural Science Foundation of Shandong Province [ZR2018ZC0129]
- Key Research and Development Plan of Jining City, P. R. China [2018HMNS001]
- P. R. China
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A metal-free polymer nanozyme has been developed for the first time simply via the one-pot covalent reaction of hyperbranched polyethyleneimine (PEI) and redox dihydroxybenzaldehyde (DHB). The yielded PEI-DHB nanoparticles could display the peroxidase-like catalysis activity, so as to act as the metal-free nanozyme in catalyzing the chromogenic reactions of 3,3',5,5'-tetramethylbenzidine and H2O2. More interestingly, the catalysis activity of the polymer nanozymes could be specifically inhibited by heparin oppositely charged. A facile visualized colorimetric method was thereby developed for sensing heparin through combining the specific catalysis and electrostatic attraction of PEI-DHB nanozyme. It was found that the developed colorimetric method can enable the detection of heparin in serum over the linear concentration range of 0.050-1.0 mu M, promising the wide applications for the detection of heparin in the clinical laboratory for the disease diagnostics and pharmacological monitoring. More importantly, such a green synthesis method may be tailored for fabricating various metal-free polymer nanozymes for the wide catalysis applications.
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