Journal
MICROCHEMICAL JOURNAL
Volume 145, Issue -, Pages 648-654Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.microc.2018.11.031
Keywords
Metal composite; Spectral analysis; Hepatotoxins; Aptamer; Conformational change
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Funding
- National Natural Science Foundation of China [21575073]
- Applied Basic Research Plan Project of Datong City, China [2018158]
- Laoshan Scholar Program of Qingdao University of Science and Technology
- Open Project Program of the Key Laboratory of Marine Bioactive Substance and Modern Analysis Technology, SOA [MBSMAT-2015-04]
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In this work, a chemiluminescence (CL) aptasensor was designed for detection of microcystin-RR (MC-RR) by introducing Cu/Co nanorods acting as CL catalyzer. Cu/Co nanorods were firstly synthesized throughout a hydrothermal method. Then the thiolated MC-RR aptamer was immobilized on the surface of Cu/Co nanorods, which was employed for recognizing of MC-RR target. In the presence of MC-RR, the conformational change of MC-RR aptamer was happened via recognizing between MC-RR and its aptamer. The luminol CL was catalyzed by Cu/Co nanorods that influenced by conformational change of MC-RR aptamer. The formed MC-RR/aptamer composite prevented the diffusion channel of luminol substrate toward the Cu/Co nanorods surface and restrained the Cu/Co nanorods catalyzing luminol CL. And the intensity of CL depended on the concentration of MC-RR. Under optimal condition, the CL aptasensor had a linear range in 0.1 to 70 nM and a low detection limit of 3.3 x 10(-11)M for MC-RR. This study provided a significant method for MC-RR detection in real samples with excellent selectivity.
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