Journal
ANALYTICAL BIOCHEMISTRY
Volume 493, Issue -, Pages 11-13Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2015.09.022
Keywords
Biosensor; Pesticide detection; Transcription factor; Gene expression; Aldehyde tag
Funding
- Chulabhorn Research Institute
- Center of Excellence on Environmental Health and Toxicology, Thailand
- Royal Golden Jubilee Ph.D. Program [PHD/0144/2554]
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The Sinorhizobium meliloti chpA promoter is highly induced in the presence of the pesticide chlorpyrifos (CPF) through the action of the transcriptional activator, ChpR. A whole-cell biosensor for the detection of CPF was developed and is composed of an Escherichia coli strain carrying a chpR expression vector and a chpA promoter-atsBA transcriptional fusion plasmid encoding sulfatase (atsA) and formylglycine generating enzyme (atsB) from Klebsiella sp. The sulfatase is posttranslationally activated by formylglycine generating enzyme (FGE) and then converts 4-methylumbelliferyl sulfate (4-MUS) to the fluorescent product, 4-methyllumbelliferone (4-MU). This biosensor system exhibited a linear response range from 25 to 500 nM CPF. (C) 2015 Elsevier Inc. All rights reserved.
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