Journal
ANALYTICAL BIOCHEMISTRY
Volume 500, Issue -, Pages 60-62Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2016.02.008
Keywords
RNA; Pancreas; Mouse; RNA purification; RNA integrity number
Funding
- Fondation contre le Cancer (Belgium)
- Universite catholique de Louvain
- Centre du Cancer (Cliniques universitaires St-Luc)
- COST action BM1204 EU_Pancreas
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Extracting RNA from pancreatic tissue is notoriously challenging because of the organ's high RNase content. Standard methods using TriPure or TRIzol classically yield RNA of sufficient quality for routine gene expression analysis but not for microarray or deep sequencing analysis. Here we developed a simple method to extract high-quality RNA from mouse pancreas. Our method uses an RNase inhibitor and combines different protocols using guanidium thiocyanate-phenol extraction. It enables reproducible isolation of RNA with an RNA integrity number around 9. (C) 2016 Elsevier Inc. All rights reserved.
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