Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 408, Issue 9, Pages 2303-2307Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-016-9326-1
Keywords
Metabolite analysis; Detergent; Tween 80; Mass spectrometry; Liquid chromatography
Funding
- Chr. Hansen A/S
- University of Copenhagen
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The presence of Polysorbate 80 in samples can challenge liquid chromatography-electrospray ionisation mass spectrometry (LC-ESI-MS) analysis as it is easily ionised and detected. In this study, we demonstrate that interference from Polysorbate 80 can be reduced by complexation with a metal ion followed by precipitation by thiocyanate. The precipitation procedure was tested on a mixture of low molecular weight compounds (e.g. amino acids and non-amino organic acids) and it was shown that none of the tested compounds were precipitated.
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