Journal
MARINE GENOMICS
Volume 46, Issue -, Pages 66-69Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.margen.2019.01.004
Keywords
Single-cell genomics; SAG; SEEP-SRB; Sulfate-reducing bacteria; Hydrocarbon degradation
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Funding
- Danish National Research Foundation [DNRF104]
- ERC Advanced Grant MICROENERGY under the European Union 7th Framework Program [294200]
- Marie Curie IIF fellowship ATP_adapt_low_energy under the European Union 7th Framework Program
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The SEEP-SRB1 is a diverse Glade of sulfate reducing bacteria affiliated with the deltaproteobacterial family Desulfobacteraceae. Here we report the draft genome sequences of two single cells belonging to the SEEP-SRB1 Glade, isolated from marine sediment in Aarhus Bay, Denmark, by fluorescence activated cell sorting (FACS). The draft genomes measure 2.12 Mb and 0.46 Mb, with GC contents of 45% and 42%. Phylogenetic placement of the 16S rRNA genes places the SAGs within the subgroups SEEP-SRB1e and SEEP-SRB1d. While these subgroups are thought to play a role in non-methane hydrocarbon degradation, there is little genomic information available to confirm their metabolic function. As such, these novel single-cell amplified genomes provide valuable insight into the metabolic potential of this prevalent subseafloor clade.
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