Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 408, Issue 22, Pages 6045-6052Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-016-9750-2
Keywords
ELISA; Clenbuterol; Hapten synthesis; beta(2)-agonist
Funding
- National Foundation for Science and Technology Development (NAFOSTED) [104.04-2014.36]
- Ho Chi Minh People's Committee
- ADS (Australian Development Scholarship)
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This research outlines the application of an enzyme-linked immunosorbent assay (ELISA) for the analysis of clenbuterol in animal products. Our assay showed good sensitivity for clenbuterol (0.4 ng/g or 0.4 ppb) and low detection limit (0.09 ng/g or 0.09 ppb). A low cross-reactivity for other beta(2)-agonist drugs such as salbutamol, terbutaline, and epinephrine led to formatting an ELISA kit considered to have a high specificity for clenbuterol. A survey of Ho Chi Minh City pork market was conducted as part of the validation of our ELISA. ELISA results showed a surprisingly high value of contamination. However, it will be necessary to conduct a more statistically valid replicated survey with evaluation by other instrumental methods to obtain a definite conclusion. This ELISA kit will be used to monitor growth promoter residues in Vietnam's animal products.
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