4.5 Article

Adenovirus type 36 regulates adipose stem cell differentiation and glucolipid metabolism through the PI3K/Akt/FoxO1/PPARγ signaling pathway

Journal

LIPIDS IN HEALTH AND DISEASE
Volume 18, Issue -, Pages -

Publisher

BMC
DOI: 10.1186/s12944-019-1004-9

Keywords

Adenovirus type 36; Adipose stem cell; Cell differentiation; Glucose and lipid metabolism; PI3K/Akt/FoxO1/PPAR gamma signaling pathway

Funding

  1. National Natural Science Foundation of China [81660154, 81460162]
  2. Doctor Research Start Fund of Xinjiang Medical University
  3. Key Discipline of the 13th Five-Year Plan (Plateau Discipline) of Xinjiang Uygur Autonomous Region

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Background: This study aims to investigate the molecular mechanism of Adenovirus type 36 (Ad36) in adipocyte differentiation and glucolipid metabolism. Methods: Rat obesity model was established by Ad36 infection and high-fat diet, respectively. Comparison of the body weight, clinical biochemical indicators, insulin sensitivity and lipid heterotopic deposition between these two models was performed. Ad36-induced adipocyte in vitro model was also established. The binding rate of FoxO1, PPAR gamma and its target gene promoter was detected using ChIP. The mRNA and protein expression levels of PPAR gamma and downstream target genes were detected by RT-PCR and Western blot, respectively. Oil red O staining was used to measure differentiation into adipocyte. Wortmannin (WM), inhibitor of PI3K, was used to act on Ad36-induced hADSCs. Results: Ad36-induced obese rats did not exhibit disorders in blood glucose and blood TG, insulin resistance and lipid ectopic deposition. The expression of Adipoq, Lpin1 and Glut4 in the adipose tissue increased. Oil red O staining showed that Ad36 induced the differentiation of hAMSCs into human adipocytes in vitro. During this process, the binding rate of FoxO1 and PPAR gamma promoter regions was weakened. However, the binding rate of the transcription factor PPAR gamma to its target genes Acc, Adipoq, Lpin1 and Glut4 was enhanced, and thus increased the protein expression of P-FoxO1, PPAR gamma 2, ACC, LPIN1, GLUT4 and ADIPOQ. The PI3K inhibitor Wortmannin reduced the expression of P-Akt, P-FoxO1 and PPAR gamma 2, thereby inhibiting adipogenesis of hADSC. Conclusion: Ad36 may promote fatty acid and triglyceride synthesis, and improve insulin sensitivity by affecting the PI3K/Akt/FoxO1/PPAR gamma signaling pathway.

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