Journal
ANALYTICA CHIMICA ACTA
Volume 920, Issue -, Pages 86-93Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2016.03.029
Keywords
NBD; Fluorescent probe; Intracellular pH; Cell imaging; Lysosomes
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Funding
- Natural Science Foundation of Shandong Province [ZR2014BM004]
- Foundation of Talent Training of Fundamental Subject of China [J1103314]
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NBDlyso with lysosome-locating morpholine moiety has been developed as a high selective and sensitive fluorescent pH probe. This probe can respond to acidic pH (2.0-7.0) in a short time (less than 1 min) and not almost change after continuously illuminated for an extended period by ultraviolet light. The fluorescence intensity of NBDlyso enhanced 100-fold in acidic solution, with very good linear relationship (R-2 = 0.996). The pK(a) of probe NBDlyso is 4.10. Therefore, NBDlyso was used to detect lysosomal pH changes successfully. Besides, X-ray crystallography was used to verify the structure of NBDlyso, and the recognition mechanism involving photo-induced electron transfer was interpreted theoretically by means of DFT and TDDFT calculations skillfully when NBDlyso comes into play under the acidic condition. This probe showed good ability to sense pH change in living cell image. (C) 2016 Elsevier B.V. All rights reserved.
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