Journal
ANALYTICA CHIMICA ACTA
Volume 920, Issue -, Pages 63-71Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2016.02.025
Keywords
Magnetic carbon dots; Staphylococcus aureus; Escherichia coli; Fluorescence detection; MALDI-MS
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Funding
- Ministry of Science and Technology of Taiwan
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A novel and facile approach of pathogenic bacteria detection, which utilizes fluorescent sensing and bacteria capture with Magnetic carbon dots (Mag-CDs), was proposed in this work. Magnetic nanoparticles were synthesized and then decorated with C-dots, and further functionalized with amine groups (chitosan). In this way, bacteria were strongly anchored on the hybrid material Mag-CDs for highly sensitive fluorescent detection. The Mag-CDs were characterized by UVevis, FT-IR spectra, TEM images, XRD, and EDX. The characterizations validate the fabrication of amine-Mag-CDs and the promising applications of this material. Fluorescence spectroscope and MALDI-MS were used for the detection and identification of bacterial strains, respectively. The limit of detection for Staphylococcus aureus and Escherichia coli was found to be 3 x 10(2) and 3.5 x 10(2) cfu mL(-1), respectively. With these encouraging results, it is expected that it would open revenues for promising applications of Mag-CDs nanomaterial. (C) 2016 Elsevier B.V. All rights reserved.
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