Journal
ANALYTICA CHIMICA ACTA
Volume 915, Issue -, Pages 74-80Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2016.02.008
Keywords
Gold nanoparticles; Peroxidase-like activity; Urea; Urease; Acetohydroxamic acid
Categories
Funding
- National Natural Science Foundation of China [21175023, 81371902]
- Program for New Century Excellent Talents in University [NCET-12-0618]
- Medical Elite Cultivation Program of Fujian Province [2013-ZQN-ZD-25]
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Herein, we reported for the first time that gold nanoparticles-catalyzed 3,3',5,5'-tetramethylbenzidine-H2O2 system can serve as an ultrasensitive colorimetric pH indicator. Gold nanoparticles acted as a catalyst and imitated the function of horseradish peroxidase. The absorbance at 450 nm of the yellow-color product in the catalytic reaction exhibited a linear fashion over the pH range of 6.40-6.60. On the basis of this property, we constructed a novel sensing platform for the determination of urea, urease, and urease inhibitor. The limit of detection for urea and urease was 5 mM and 1.8 U/L, respectively. The half-maximal inhibition value IC50 of acetohydroxamic acid was found to be 0.05 mM. Urea in human urine and urease in soil were detected with satisfied results. (C) 2016 Elsevier B.V. All rights reserved.
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