Journal
ANALYTICA CHIMICA ACTA
Volume 948, Issue -, Pages 55-61Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2016.10.038
Keywords
DOM; SPE; ESI-FT-ICR-MS; Stepwise elution
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Funding
- 973 Program of China [2014CB441102]
- Strategic Priority Research Program of the Chinese Academy of Sciences [XDB14020202]
- National Natural Science Foundation of China [21407161, 21321004, 21537005]
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Characterization of dissolved organic matter (DOM) at the molecular level will greatly improve our understanding of its bio-geochemical role in controlling the fate of contaminants in the environment, and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) is the most powerful analytical technique for this purpose. Before FT-ICR-MS analysis, isolation, desalination and concentration of DOM are necessary, and solid-phase extraction (SPE) is the most widely applied pretreatment procedure. However, some molecular information is lost using conventional SPE methods. Here, we propose a novel strategy of SPE enrichment using stepwise elution (SPE-SE). Compounds in DOM were divided into three fractions by this SPE-SE procedure according to their polarity and ionization efficiency. The diversity of DOM molecules identified by ESI-FT-ICR-MS using SPE-SE exceeded those using conventional SPE methods by more than 50%. This method is feasible and has the potential to be used as a pretreatment strategy for complex DOM matrixes prior to ESI-FT-ICR-MS analysis, especially for those rich in nitrogenous molecules, carbohydrates, lipids and/or aromatic compounds. (C) 2016 Elsevier B.V. All rights reserved.
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