Journal
ANALYTICA CHIMICA ACTA
Volume 948, Issue -, Pages 90-97Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2016.10.031
Keywords
Nitrogen-doped graphene quantum dots; Silver nanoparticles; Fluorescence resonance energy transfer; Homogeneous assay; Transgenic soybean
Categories
Funding
- National Natural Science Foundation of China [21375050, 21405063, 21505055, 21675066]
- Priority Academic Program Development of Jiangsu Higher Education Institutions [PAPD-2014-37]
- Senior Professional Research Start-up Fund of Jiangsu University [5503000011]
- Natural Science Foundation of the Jiangsu Higher Education Institutions, China [15KJA550001]
- Innovation Project of Science and Technology for College Graduates of Jiangsu Province [KYLX15_1091]
- China Postdoctoral Science Foundation [2015M581745, 2015M580401]
- Jiangsu Province Postdoctoral Science Foundation [1501108C]
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In this work, a novel homogeneous assay for DNA quantitative analysis based on forster resonance energy transfer (FRET) was developed for cauliflwer mosaic virus 35s (CaMV35S) promoter of transgenic soybean detection. The homogenous FRET of fluorescence signal was fabricated by DNA hybridization with probe modified nitrogen-doped graphene quantum dots (NGQDs) and silver nanoparticles (AgNPs), which acted the donor-acceptor pairs for the first time. The highly efficient FRET and unique properties of the NGQDs made the proposed FRET system as a functionalized detection platform for labelling of DNA. Upon the recognition of specific target DNA (tDNA), the FRET between NGQDs and AgNPs was triggered to produce fluorescence quenching, which could be used for tDNA detection. The fabricated homogeneous FRET assay displayed a wide linear range of 0.1-500.0 nM and a low limit of detection 0.03 nM for the detection of CaMV35S (S/N = 3). This proposed biosensor revealed high specificity to detect tDNA, with acceptable intra-assay precision and excellent stability. This method was successfully applied to identify the real sample of 0.5% containing transgenic soybean, which achieved the most of national law regulations. This assay was further validated by polymerase chain reaction as the genetically modified organisms, suggesting that the proposed FRET system is a feasible tool for the further daily genetically modified organism detection. (C) 2016 Elsevier B.V. All rights reserved.
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