Journal
ANALYST
Volume 141, Issue 16, Pages 4961-4967Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c6an00957c
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Funding
- National Basic Research Program of China (973 program) [2011CB911003]
- National Natural Science Foundation of China [21275069, 21577057]
- Analysis & Test Fund of Nanjing University
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A novel strategy for the preparation of an aptamer based organic-inorganic hybrid affinity monolithic column was developed successfully using gold nanoparticles (GNPs) as an intermediary for a sandwich structure to realize the functional modification of the surface of the monolithic matrix. This monolithic matrix was facilely pre-synthesized via one-step co-condensation. Due to the high surface-to-volume ratio of GNPs and the large specific surface area of the hybrid matrix, the average coverage density of aptamers on the hybrid monolith reached 342 pmol mu L-1. With the combination of an aptamer based hybrid affinity monolithic column and enzymatic chromogenic assay, the quantitation and detection limits of thrombin were as low as 5 nM and 2 nM, respectively. These results indicated that the GNPs attached monolith provided a novel technique to immobilize aptamers on an organic-inorganic hybrid monolith and it could be used to achieve highly selective recognition and determination of trace proteins.
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