Journal
ANALYST
Volume 141, Issue 12, Pages 3621-3625Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5an02554k
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Funding
- National Natural Science Foundation of China [21205077, 81373621]
- Youth Program of the National 1000 Talents Project
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We describe a one-step microfludics-based immunomagnetic isolation method to isolate CTCs directly from the whole blood of lung adenocarcinoma patients. This method avoids harsh sample preparation and enrichment steps, and therefore preserves the viability of CTCs during the in vitro isolation. Importantly, isolated, magnetic bead-bearing CTCs are concentrated in a small volume of culture medium with a high CTC density. High cell viability and culturing density promote the ex vivo expansion of limited numbers of CTCs. Expanded CTCs are characterized at the genetic, protein and metabolic levels.
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