Journal
ANALYST
Volume 141, Issue 3, Pages 1105-1111Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5an02163d
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Funding
- National Natural Science Foundation of China [31501564, 31471639]
- National Key Technologies R & D Program of China during the 12th Five-Year Plan Period [2012BAD29B06]
- Science & Technology Department of Liaoning Province of China [2015103020]
- Team Support Program for the Taishan Scholar of Blue Industry leading personnel of Shandong Province of China [LZBZ2015-19]
- Open Foundation of Food Safety Key Laboratory of Liaoning Province [LNSAKF2013012]
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An optical biosensing method using CdTe quantum dots (QDs) and bi-enzyme-immobilized eggshell membranes for the determination of organophosphorus pesticides (OPs) has been developed. Increasing amounts of OPs led to a decrease of the enzymatic activity and thus a decrease in the production of hydrogen peroxide (H2O2), which can quench the fluorescence of the CdTe QDs. Under the optimum conditions, there was a good linear relationship between the enzyme inhibition percentage and the logarithm of paraoxon or parathion concentration in the range of 1.0 x 10(-11)-1.0 x 10(-6) mol L-1. The detection limit (S/N = 3) of the proposed biosensors were as low as 4.30 x 10(-12) mol L-1 for paraoxon and 2.47 x 10(-12) mol L-1 for parathion. The bi-enzyme-immobilized eggshell membrane demonstrated a long shelf-life of at least 2 months and the results showed good repeatability. The proposed method was successfully applied to the determination of the OPs in real fruit samples with satisfactory results.
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