Journal
JOURNAL OF MOLECULAR LIQUIDS
Volume 284, Issue -, Pages 215-224Publisher
ELSEVIER
DOI: 10.1016/j.molliq.2019.03.152
Keywords
Polyelectrolytes; Biodegradable capsules; Dextran sulfate; Poly-L-arginine; Layer-by-layer (LbL) assembly; Temperature induced shrinking; MCF-7 cells; MCF-7/ADR cells; Drug resistance
Funding
- Russian Foundation for Basic Research [017-33-80141 mol_ev_a]
- Ministry of Science and Higher Education
- Russian Science Foundation [18-19-00718]
- Russian Science Foundation [18-19-00718] Funding Source: Russian Science Foundation
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Doxorubicin (DOX) is one of mainstays in anticancer therapy due to its universal anticancer activity. Development of DOX delivery systems is of particular importance aiming a reduction in its non-targeted toxicity and improvement of the treatment efficiency. In this study, biodegradable polyelectrolyte multilayer capsules from a dextran sulfate (DS) and poly-L-arginine (Parg) couple for intracellular DOX delivery has been developed. The capsules were fabricated by layer-by-layer (LbL) technique using vaterite particles (500 +/- 100 nm) as sacrificial templates. An elegant approach for simultaneous miniaturization of the capsules down to a size of 290 +/- 60 nm and DOX loading was elaborated. Cytotoxicity of the DOX-loaded capsules was evaluated in vitro using human breast adenocarcinoma MCF-7 and DOX-resistant MCF-7/ADR cells. Penetration and accumulation of the capsules in the cells were studied by confocal microscopy and flow cytometry. The pronounced accumulation of the DOX-loaded shrunken capsules in MCF-7/ADR cells suggested overcoming drug resistance. The developed capsules could be promising for both anticancer therapy and diagnostic purposes. (C) 2019 Elsevier B.V. All rights reserved.
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