Journal
JOURNAL OF MEDICINAL CHEMISTRY
Volume 62, Issue 7, Pages 3677-3695Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jmedchem.9b00164
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Funding
- NIDDK Intramural Research Program [ZIADK031116]
- Deutsche Forschungsgemeinschaft (German Research Foundation) [JU 2966/1-1]
- Cells in -Motion (CiM) cluster of excellence (Munster, Germany)
- NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES [ZIADK031117] Funding Source: NIH RePORTER
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Cluster of differentiation 73 (CD73) converts adenosine 5'-monophosphate to immunosuppressive adenosine, and its inhibition was proposed as a new strategy for cancer treatment. We synthesized 5'-O-[(phosphonomethyl)phosphonic acid] derivatives of purine and pyrimidine nucleosides, which represent nucleoside diphosphate analogues, and compared their CD73 inhibitory potencies. In the adenine series, most ribose modifications and 1-deaza and 3-deaza were detrimental, but 7-deaza was tolerated. Uracil substitution with N-3-methyl, but not larger groups, or 2-thio, was tolerated. 1,2-Diphosphono-ethyl modifications were not tolerated. N-4-(Aryl)alkyloxy-cytosine derivatives, especially with bulky benzyloxy substituents, showed increased potency. Among the most potent inhibitors were the 5'-O-[(phosphonomethyl)phosphonic acid] derivatives of S-fluorouridine (41), N-4-benzoyl-cytidine (7f), N-4-[O-(4-benzyloxy)]-cytidine (9h), and N-4-[O-(4-naphth-2-ylmethyloxy)]-cytidine (9e) (K-i values 5-10 nM at human CD73). Selected compounds tested at the two uridine diphosphate-activated P2Y receptor subtypes showed high CD73 selectivity, especially those with large nucleobase substituents. These nucleotide analogues are among the most potent CD73 inhibitors reported and may be considered for development as parenteral drugs.
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