Journal
JOURNAL OF IMMUNOLOGICAL METHODS
Volume 465, Issue -, Pages 45-52Publisher
ELSEVIER
DOI: 10.1016/j.jim.2018.12.004
Keywords
Phage display; M13 filamentous phage; Sepsis; Phage-capture system; Raman spectroscopy
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Funding
- Italian Ministry of Education, University and Research (MIUR) [PON02 00355]
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Sepsis is a systemic inflammatory response ensuing from presence and persistence of microorganisms in the bloodstream. The possibility to identify them at low concentrations may improve the problem of human health and therapeutic outcomes. So, sensitive and rapid diagnostic systems are essential to evaluate bacterial infections during the time, also reducing the cost. In this study, from random M13 phage display libraries, we selected phage clones that specifically bind surface of Staphyloccocus aureus, Pseudomonas aeruginosa and Escherichia coll. Then, commercial magnetic beads were functionalized with phage clones through covalent bond and used as capture and concentrating of pathogens from blood. We found that phage-magnetic beads complex represents a network which enables a cheap, high sensitive and specific detection of the bacteria involved in sepsis by micro-Raman spectroscopy. The enter process required 6 h and has the limit of detection of 10 Colony Forming Units on 7 ml of blood (CFU/7 ml).
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