Application of the Mechanical High-Pressure Method Combined with High-Performance Liquid Chromatography-Tandem Mass Spectrometry for Determination of Veterinary Drug Residues in Incurred Chicken and Rabbit Muscle Tissues

u Rapid sample preparation is a key step in the field of food safety. A mechanical high-pressure method using a laboratory-made meat press machine was first introduced in this study to process the incurred muscle samples of chicken and rabbit. By applying high pressure to animal muscle, the meat juice was obtained. After extraction and purification, veterinary drug residues in the juice were qualitatively and quantitatively analyzed by using high-performance liquid chromatography-tandem mass spectrometry. The sample press conditions and extraction solvents were optimized. Under the optimal conditions, all veterinary drug residues, including tetracycline, enrofloxacin, clenbuterol, ampicillin, lincomycin, erythromycin, and sulfadiazine, in the incurred samples were detected. The residual concentration of drugs in samples obtained by using the mechanical high-pressure method can reach up to 94.0% of that obtained by using the common homogenization method, suggesting that drug residues exist in the tissue juice, which justifies the use of the mechanical high-pressure method. Moreover, with the mechanical high-pressure method, the sample preparation time was shortened by five times, and the consumption of the extraction solvent was reduced by 50%, relative to the homogenization method.