Journal
JOURNAL OF FOOD BIOCHEMISTRY
Volume 43, Issue 4, Pages -Publisher
WILEY
DOI: 10.1111/jfbc.12800
Keywords
ACE-inhibitory activity; antioxidant activity; bioactive peptides; proteinase K; wheat germ protein
Funding
- University of Tabriz (Tabriz, I.R. IRAN)
- R&D Institute of Novin Daneshmand (Tehran, Iran)
- Spanish Ministry of Economy and Competitiveness (MINECO) [AGL2014-52395-C2-2-R]
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Wheat germ protein hydrolysate (WGPH) was obtained by proteinase K digestion, in order to produce bioactive antioxidant and antihypertensive peptides. Response surface methodology (RSM) was used to optimize hydrolysis conditions (enzyme-to-substrate ratio, time, and temperature) for antioxidant activity of hydrolysates. The crude WGPH produced in this way significantly inhibited angiotensin-I converting enzyme (ACE) in a concentration-dependent manner. It was next fractionated by reversed-phase semi-preparative High Performance Liquid Chromatography (HPLC) into 12 fractions that were examined for antioxidant and antihypertensive activities. Fractions with antioxidant and ACE-inhibitory activities were then submitted to further analysis by nano-LC-ESI-MS-MS. Among the various peptides identified, MDATALHYENQK (IC50: 293.3 +/- 6.5 mu g/ml) and SGGSYADELVSTAK (IC50: 265.5 +/- 8.3 mu g/ml) displayed antioxidant activity and VALTGDNGHSDHVVHF (IC50: 189.3 +/- 4.05 mu g/ml), VDSLLTAAK (IC50: 159.7 +/- 0.33 mu g/ml), MDATALHYENQK (IC50: 303.6 +/- 2.47 mu g/ml), IGGIGTVPVGR (IC50: 125.7 +/- 2.3 mu g/ml) and SGGSYADELVSTAK (IC50: 128.2 +/- 1.17 mu g/ml) showed good ACE-inhibitory activity. Practical applications Wheat milling industries produce massive amounts of wheat germ as by-product that can be converted into valuable compounds. The present research indicates that proteinase K is useful to hydrolyze wheat germ proteins in a search for bioactive peptides with antioxidant and ACE-inhibitory properties. The identified peptides can be regarded as functional food additives, or nutraceuticals to improve human health.
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