Journal
JOURNAL OF EXPERIMENTAL BOTANY
Volume 70, Issue 8, Pages 2325-2338Publisher
OXFORD UNIV PRESS
DOI: 10.1093/jxb/erz023
Keywords
Arabidopsis; chloroplast; dual localization; endoplasmic reticulum (ER); ER localization; energy status; Nicotiana benthamiana; nuclear localization; retrograde signalling; SnRK1.1
Categories
Funding
- Swedish Research Council, VR
- STINT
- Australian Research Council [CE 140100008]
- ICGEB (SMART Fellowship)
- FONCyT [PICT2016-0093]
- CONICET
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Sucrose non-fermenting 1 (SNF1)-related protein kinase 1.1 (SnRK1.1; also known as KIN10 or SnRK1 alpha) has been identified as the catalytic subunit of the complex SnRK1, the Arabidopsis thaliana homologue of a central integrator of energy and stress signalling in eukaryotes dubbed AMPK/Snf1/SnRK1. A nuclear localization of SnRK1.1 has been previously described and is in line with its function as an integrator of energy and stress signals. Here, using two biological models (Nicotiana benthamiana and Arabidopsis thaliana), native regulatory sequences, different microscopy techniques, and manipulations of cellular energy status, it was found that SnRK1.1 is localized dynamically between the nucleus and endoplasmic reticulum (ER). This distribution was confirmed at a spatial and temporal level by co-localization studies with two different fluorescent ER markers, one of them being the SnRK1.1 phosphorylation target HMGR. The ER and nuclear localization displayed a dynamic behaviour in response to perturbations of the plastidic electron transport chain. These results suggest that an ER-associated SnRK1.1 fraction might be sensing the cellular energy status, being a point of crosstalk with other ER stress regulatory pathways.
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