Journal
JOURNAL OF CELLULAR BIOCHEMISTRY
Volume 120, Issue 8, Pages 13168-13176Publisher
WILEY
DOI: 10.1002/jcb.28591
Keywords
cytoplasmic redistribution; hypo-osmosis; intermediate filaments; osmotic stress; vimentin
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Funding
- 100 talents program from the Chinese Academy of Sciences
- CAS Key Laboratory of Molecular Virology & Immunology, Institut Pasteur of Shanghai [KLMVI-OP-201904]
- Institut Pasteur of Shanghai, Chinese Academy of Sciences [1185170000]
- Sigrid Juselius foundation
- National Natural Science Foundation of China [81702750]
- Basic Research Project of Shenzhen [JCY20170818164756460]
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Intermediate filaments (IFs) play a key role in the control of cell structure and morphology, cell mechano-responses, migration, proliferation, and apoptosis. However, the mechanisms regulating IFs organization in motile adhesive cells under certain physical/pathological conditions remain to be fully understood. In this study, we found hypo-osmotic-induced stress results in a dramatic but reversible rearrangement of the IF network. Vimentin and nestin IFs are partially depolymerized as they are redistributed throughout the cell cytoplasm after hypo-osmotic shock. This spreading of the IFs requires an intact microtubule network and the motor protein associated transportation. Both nocodazole treatment and depletion of kinesin-1 (KIF5B) block the hypo-osmotic shock-induced rearrangement of IFs showing that the dynamic behavior of IFs largely depends on microtubules and kinesin-dependent transport. Moreover, we show that cell survival rates are dramatically decreased in response to hypo-osmotic shock, which was more severe by vimentin IFs depletion, indicating its contribution to osmotic endurance. Collectively, these results reveal a critical role of vimentin IFs under hypotonic stress and provide evidence that IFs are important for the defense mechanisms during the osmotic challenge.
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