Journal
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
Volume 40, Issue 24, Pages 13355-13365Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/07391102.2019.1592024
Keywords
Protein kinases; eEF-2K; homology modeling; molecular docking; molecular dynamics simulation
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Funding
- Scientific and Technological Research Council of Turkey TUBITAK [215S008]
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Protein kinases are successful drug targets, and eEF-2K has been identified as a potential therapeutic target for certain aggressive solid cancers. This study used modeling, docking analysis, and molecular dynamics simulations to understand the binding mechanism of eEF-2K with inhibitors, providing insights for the development of new selective and potential eEF-2K inhibitors.
Protein kinases emerged as one of the most successful families of drug targets due to their increased activity and involvement in mediating critical signal transduction pathways in cancer cells. Recent evidence suggests that eukaryotic elongation factor 2 kinase (eEF-2K) is a potential therapeutic target for treating some highly aggressive solid cancers, including lung, pancreatic and triple-negative breast cancers. Thus, several compounds have been developed for the inhibition of the enzyme activity, but they are not sufficiently specific and potent. Besides, the crystal structure of this kinase remains unknown. Hence, the functional organization and regulation of eEF-2K remain poorly characterized. For this purpose, we constructed a homology model of eEF-2K and then used docking methodology to better understanding the binding mechanism of eEF-2K with 58 compounds that have been proposed as existing inhibitors. The results of this analysis were compared with the experimental results and the compounds effective against eEF-2K were determined against eEF-2K as a result of both studies. And finally, molecular dynamics (MD) simulations were performed for the stability of eEF-2K with these compounds. According to these study defined that the binding mechanism of eEF-2K with inhibitors at the molecular level and elucidated the residues of eEF-2K that play an important role in enzyme selectivity and ligand affinity. This information may lead to new selective and potential drug molecules to be for inhibition of eEF-2K.
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