Journal
JOURNAL OF BIOMEDICAL OPTICS
Volume 24, Issue 3, Pages -Publisher
SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
DOI: 10.1117/1.JBO.24.3.035003
Keywords
optical parameters; refractive index; dispersion; biotissue; total internal reflection method
Funding
- National Key R&D Program of China [2018YFF0212105]
- Natural Science Foundation of China [61475078]
- National Natural Science Foundation [31527801]
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The refractive index of blood is a key biophysical parameter, which can reflect the physiological state. We measured the refractive index of whole blood and other components, such as serum, plasma, and hemoglobin, based on internal reflection by using a homemade apparatus in the spectral range of 400 to 750 nm. In addition to the hemoglobin solution, which has a Soret band about 420 nm and two Q-bands between 500 and 600 nm, the measurements of other samples are the normal dispersion curve. The results are approximated by the Cauchy equation and Sellmeier equation, and the correlation coefficients are more than 0.997. (C) The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License.
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