Journal
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
Volume 108, Issue 1, Pages 128-142Publisher
WILEY
DOI: 10.1002/jbm.b.34372
Keywords
nerve conduit; polyaniline; debris; nerve regeneration; reactive oxygen species
Funding
- International S&T Cooperation Program of China [2014DFG02330, 2015DFG32730]
- Science and Technology Commission Shanghai Municipality [15540723900, 18490740200]
- Shanghai Municipal Education Commission
- Gaofeng Biomedical Engineering Grant [ZXGF082101]
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Endowing the conduit with conductivity has been an effective way to stimulate nerve growth and functional recovery. Here, conducting polyaniline (PANi) was used to construct a conductive guidance by coating on the surface of microtubes inserted in a three-dimensional zein nerve conduit to study the repairing efficacy on peripheral nerve injury. PANi nanoparticles with a size of 20-30 nm were synthesized and coated on the surface of microtubes through layer-by-layer deposition. Then, conduits including microtubes with and without PANi coating were implanted into rats to bridge a 10-mm sciatic nerve defect and autograft as the control group. After 2 months, the conduit with PANicoating improved the recovery of proximal compound muscle action potential significantly in the regenerated nerve compared to the conduit without PANi coating, which was not inferior to the autograft group. However, the repairing efficacy was changed reversely at the fourth month postimplantation. PANi coating fragmented to form debris within or around the regenerated nerves while microtubes seem to degrade completely as observed by H&E staining. In vitro degradation experiment confirmed this process. The PANi nanoparticles could induce cytotoxicity and reactive oxygen species (ROS) generation of both NIH 3T3 cells and macrophage cell line RAW 264.7. These in vitro and in vivo results implied that the nondegradable PANi may occupy the regeneration space and stimulate the inflammatory response in later implantation in vivo. While there was no such risk if the PANi coating keeps in an intact film. (c) 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 108B:128-142, 2020.
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