Journal
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
Volume 33, Issue 6, Pages -Publisher
WILEY
DOI: 10.1002/jbt.22303
Keywords
cytotoxicity; Fe2O3 NPs; lysosomes; mitochondria; oxidative stress (OS)
Categories
Ask authors/readers for more resources
Magnetic nanoparticles (NPs) are used to a large extent in the targeted delivery of therapeutic agents. In this study, we aimed to investigate the possible toxicity of Fe2O3 NPs on human cells, including blood lymphocytes. We isolated blood lymphocytes from healthy humans using Ficoll polysaccharide and subsequently by gradient centrifugation. Then, the toxicity parameters, including cell viability, reactive oxygen species (ROS) formation, lipid peroxidation, cellular glutathione (GSH) level, mitochondrial and lysosomal damage, were measured in blood lymphocytes after exposure to Fe2O3 NPs. Our results indicated that Fe2O3 NPs significantly (dependent on concentration) reduced the cell viability, and the IC50 was determined to be 1 mM. With increasing concentrations, we found that Fe2O3 NPs-induced cell toxicity was associated with a significant increase in intracellular ROS and loss of mitochondrial membrane potential and lysosomal membrane leakiness. Consequently, these NPs at different concentrations affect GSH level and cause oxidative stress in human lymphocytes.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available