Journal
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
Volume 74, Issue 6, Pages 1494-1502Publisher
OXFORD UNIV PRESS
DOI: 10.1093/jac/dkz080
Keywords
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Funding
- Deutsche Forschungsgemeinschaft [DFG FOR 2251, PR 1405/4-1]
- EU [643476]
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Objectives: The aim of this study was to characterize the Acinetobacter calcoaceticus clinical isolate AC_2117 with the novel carbapenem-hydrolysing class D beta-lactamase (CHDL) OXA-679. Methods: Identification of the species and beta-lactamases was verified by genome sequencing (PacBio) and phylogenetic analyses. Antibiotic susceptibility of AC_2117 and transformants harbouring cloned bla(OXA-679) was evaluated using antibiotic gradient strips and microbroth dilution. OXA-679 was purified heterologously and kinetic parameters were determined using spectrometry or isothermal titration calorimetry. The impact of OXA-679 production during imipenemtherapy was evaluated in the Galleria mellonella infection model. Results: Sequencing of the complete genome of the clinical A. calcoaceticus isolate AC_2117 identified a novel CHDL, termed OXA-679. This enzyme shared sequence similarity of 71% to each of the families OXA-143 and OXA-24/40. Phylogenetic analyses revealed that OXA-679 represents a member of a new OXA family. Cloning and expression of bla(OXA-679) as well as measurement of kinetic parameters revealed the effective hydrolysis of carbapenems which resulted in reduced susceptibility to carbapenems in Escherichia coli and A. calcoaceticus, and high-level carbapenem resistance in Acinetobacter baumannii. Infection of larvae of G. mellonella with a sublethal dose of bla(OXA-679)-expressing A. baumannii could not be cured by high-dose imipenemtherapy, indicating carbapenem resistance in vivo. Conclusions: We identified bla(OXA-679) in a clinical A. calcoaceticus isolate that represents a member of the new OXA-679 family and that conferred high-level carbapenem resistance in vitro and in vivo.
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