Two new phenazine metabolites with antimicrobial activities from soil-derived Streptomyces species

Two new phenazine metabolites, 6-hydroxyphenazine-1-carboxamide (1) and methyl 6-carbamoylphenazine-1-carboxylate (2), were isolated from a soil-derived Streptomyces diastaticus subsp. ardesiacus strain YIM PH20246, and their structures were elucidated by extensive spectroscopic data analysis. The antimicrobial activities of the isolates were assayed. Compound 1 showed moderate antifungal and antibacterial activities against Fusarium oxysporum (ATCC 7808), Fusarium solani (ATCC 36031) and Plectosphaerella cucumerina (local isolate), Staphylococcus aureus (ATCC 25923) and Staphylococcus albus (ATCC 10231), respectively. Compound 2 exhibited moderate antifungal and antibacterial activities against F. oxysporum, F. solani, and Phoma herbarum, S. aureus, S. albus, and Bacillus subtilis, respectively.