Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 67, Issue 10, Pages 3028-3036Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.8b06449
Keywords
fluorescent magnetic nanobeads; immunomagnetic separation; fluorescent lateral flow immunoassay; swine urine; clenbuterol
Funding
- National Natural Science Foundation of China [31772066]
- Free Explore Issue of State Key Laboratory of Food Science and Technology of Nanchang University [SKLF-ZZB-201719]
- Jiangxi Special Fund for Agro-scientific Research in the Collaborative Innovation [JXXTCX201703-1]
- Earmarked Fund for the Jiangxi Agriculture Research System [JXARS-03]
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The lack of sensitivity and poor matrix tolerance are the main bottlenecks of the lateral flow immunoassay (LFIA). Here, a sensitive and matrix-tolerant method that integrated immunomagnetic separation and fluorescent lateral flow immunoassay (IMS-FLFIA) based on fluorescent magnetic nanobeads was developed to detect the clenbuterol (CLE) residue in swine urine. The limit of detection (LOD) of IMS-FLFIA is 4 times lower than that of traditional colloidal gold LFIA. This method, which exhibits similar LOD and linearity range in both phosphate-buffered saline and urine swine, is highly correlated with liquid chromatography-tandem mass spectrometry for the detection of real swine urine samples. The result indicated that IMS-FLFIA has a universal resistance to the swine urine matrix. The merits of this assay, high sensitivity, matrix tolerance, accuracy, and specificity, ensure a promising future in detection of veterinary drug residues.
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